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4060 p erbb 4  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc 4060 p erbb 4
    4060 P Erbb 4, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 14811 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/4060 p erbb 4/product/Cell Signaling Technology Inc
    Average 99 stars, based on 14811 article reviews
    4060 p erbb 4 - by Bioz Stars, 2026-03
    99/100 stars

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    Alterations in ErbB4 activation after CCI. ( A and B ), Mechanical allodynia ( A ) and heat hyperalgesia ( B ) was observed in the ipsilateral hindpaw after CCI, n=8. ( C ), Time course of ErbB4 and phosphor-ErbB4 (p-ErbB4) expression in the spinal cord after chronic constriction injury (CCI) or sham surgery, the representative Western blots were shown in left and the quantitative data was shown in right, n=6. ( D ), ErbB4 activation in the spinal cord on day 7 after (CCI, SNL), and SNI, n=6.

    Journal: Journal of Pain Research

    Article Title: ErbB4 in Spinal PV Interneurons Regulates Mechanical Allodynia in Neuropathic Pain via Modulation of Glycinergic Inhibitory Tone

    doi: 10.2147/JPR.S311894

    Figure Lengend Snippet: Alterations in ErbB4 activation after CCI. ( A and B ), Mechanical allodynia ( A ) and heat hyperalgesia ( B ) was observed in the ipsilateral hindpaw after CCI, n=8. ( C ), Time course of ErbB4 and phosphor-ErbB4 (p-ErbB4) expression in the spinal cord after chronic constriction injury (CCI) or sham surgery, the representative Western blots were shown in left and the quantitative data was shown in right, n=6. ( D ), ErbB4 activation in the spinal cord on day 7 after (CCI, SNL), and SNI, n=6.

    Article Snippet: Antibodies against ErbB4, phospho-ErbB4 (p-ErbB4, Tyr 1056), and parvalbumin were purchased from Santa Cruz Biotechnology.

    Techniques: Activation Assay, Expressing, Western Blot

    Cellular localization of p-ErbB4 expression in the spinal cord. ( A and B ), Combined p-ErbB4 and NeuN (a neural marker) ( A ) or parvalbumin ( B ) immunofluorescence staining in the spinal cord on day 7 after CCI or sham surgery. Scale bar, 25 μm.

    Journal: Journal of Pain Research

    Article Title: ErbB4 in Spinal PV Interneurons Regulates Mechanical Allodynia in Neuropathic Pain via Modulation of Glycinergic Inhibitory Tone

    doi: 10.2147/JPR.S311894

    Figure Lengend Snippet: Cellular localization of p-ErbB4 expression in the spinal cord. ( A and B ), Combined p-ErbB4 and NeuN (a neural marker) ( A ) or parvalbumin ( B ) immunofluorescence staining in the spinal cord on day 7 after CCI or sham surgery. Scale bar, 25 μm.

    Article Snippet: Antibodies against ErbB4, phospho-ErbB4 (p-ErbB4, Tyr 1056), and parvalbumin were purchased from Santa Cruz Biotechnology.

    Techniques: Expressing, Marker, Immunofluorescence, Staining

    Manipulation of ErbB4 in the spinal cord regulates pain behaviors. ( A ), The validation of ErbB4 siRNA transfection efficiency in vivo, n=4. ( B and C ), Effect of ErbB4 siRNA (10μg/5μL) on the development of CCI-induced mechanical allodynia ( B ) and thermal hyperalgesia ( C ), n=8. ( D and E ), Effect of ErbB4 siRNA (10μg/5μL) on the maintenance of CCI-induced mechanical allodynia ( D ) and thermal hyperalgesia ( E ), n=8. ( F ), Phosphorylation of ErbB4 after spinal administration of NRG1 (10μM/5μL), n=6. ( G and H ), Effect of NRG1 (10μM/5μL) on the mechanical allodynia ( G ) and thermal hyperalgesia ( H ) in naïve mice, n=8. ( I and J ), Effect of ErbB4 siRNA on the NRG1-induced mechanical allodynia ( I ) and thermal hyperalgesia ( J ) in naïve mice, n=8.

    Journal: Journal of Pain Research

    Article Title: ErbB4 in Spinal PV Interneurons Regulates Mechanical Allodynia in Neuropathic Pain via Modulation of Glycinergic Inhibitory Tone

    doi: 10.2147/JPR.S311894

    Figure Lengend Snippet: Manipulation of ErbB4 in the spinal cord regulates pain behaviors. ( A ), The validation of ErbB4 siRNA transfection efficiency in vivo, n=4. ( B and C ), Effect of ErbB4 siRNA (10μg/5μL) on the development of CCI-induced mechanical allodynia ( B ) and thermal hyperalgesia ( C ), n=8. ( D and E ), Effect of ErbB4 siRNA (10μg/5μL) on the maintenance of CCI-induced mechanical allodynia ( D ) and thermal hyperalgesia ( E ), n=8. ( F ), Phosphorylation of ErbB4 after spinal administration of NRG1 (10μM/5μL), n=6. ( G and H ), Effect of NRG1 (10μM/5μL) on the mechanical allodynia ( G ) and thermal hyperalgesia ( H ) in naïve mice, n=8. ( I and J ), Effect of ErbB4 siRNA on the NRG1-induced mechanical allodynia ( I ) and thermal hyperalgesia ( J ) in naïve mice, n=8.

    Article Snippet: Antibodies against ErbB4, phospho-ErbB4 (p-ErbB4, Tyr 1056), and parvalbumin were purchased from Santa Cruz Biotechnology.

    Techniques: Biomarker Discovery, Transfection, In Vivo, Phospho-proteomics

    Inhibition of glycine release is required for the modulation of mechanical allodynia by ErbB4. ( A and B ), Effect of ErbB4 siRNA (10μg/5μL) on the release of spinal GABA and glycine in CCI mice, n=6. ( C and D ), Effect of NRG1 (10μM/5μL) on the release of spinal GABA and glycine in naive mice, n=6. ( E ), Bicuculline (0.05μg/5μL) and saclofen (5μg/5μL) have no effect on the decreased mechanical allodynia induced by ErbB4 siRNA in CCI mice, n=8. ( F ), Strychnine (1μg/5μL) reversed the decreased mechanical allodynia induced by ErbB4 siRNA in CCI mice, n=8. ( G and H ), Glycine (0.5μg/5μL), not muscimol (0.1μg/5μL) or baclofen (0.5μg/5μL), reversed the NRG1-induced mechanical allodynia in naïve mice, n=8. ( I and J ), Muscimol (0.1μg/5μL), baclofen (0.5μg/5μL), or Glycine (0.5μg/5μL) have no effect on the mechanical allodynia and thermal hyperalgesia induced by NRG1, n=8.

    Journal: Journal of Pain Research

    Article Title: ErbB4 in Spinal PV Interneurons Regulates Mechanical Allodynia in Neuropathic Pain via Modulation of Glycinergic Inhibitory Tone

    doi: 10.2147/JPR.S311894

    Figure Lengend Snippet: Inhibition of glycine release is required for the modulation of mechanical allodynia by ErbB4. ( A and B ), Effect of ErbB4 siRNA (10μg/5μL) on the release of spinal GABA and glycine in CCI mice, n=6. ( C and D ), Effect of NRG1 (10μM/5μL) on the release of spinal GABA and glycine in naive mice, n=6. ( E ), Bicuculline (0.05μg/5μL) and saclofen (5μg/5μL) have no effect on the decreased mechanical allodynia induced by ErbB4 siRNA in CCI mice, n=8. ( F ), Strychnine (1μg/5μL) reversed the decreased mechanical allodynia induced by ErbB4 siRNA in CCI mice, n=8. ( G and H ), Glycine (0.5μg/5μL), not muscimol (0.1μg/5μL) or baclofen (0.5μg/5μL), reversed the NRG1-induced mechanical allodynia in naïve mice, n=8. ( I and J ), Muscimol (0.1μg/5μL), baclofen (0.5μg/5μL), or Glycine (0.5μg/5μL) have no effect on the mechanical allodynia and thermal hyperalgesia induced by NRG1, n=8.

    Article Snippet: Antibodies against ErbB4, phospho-ErbB4 (p-ErbB4, Tyr 1056), and parvalbumin were purchased from Santa Cruz Biotechnology.

    Techniques: Inhibition